Regional Institute of Wine and Oil

New IRVO virological analysis services

Upon request from Sicilian wine producers, IRVO has activated at the technical-information desk in Canicattì, an analysis laboratory for research for virological diseases of the vine and soil nematodes. Therefore, the search for the following viruses can be carried out using ELISA tests on leaf material and shoots:

 

 

Infectious degeneration complex	Arabis mosaic virus	ArMV
Leaf curl virus	GFLV
Leaf curl complex	Curl virus Foliar 1,2 and 3	GLRa-V1
GLRa -V2
GLRa-V3
Curly wood complex	Wood groove virus	GVA
Wood pit virus	GVB
Mosaic complex	Infectious spot virus	GFKV

 

Furthermore, soil analyzes can be carried out for nematological research of Xiphinema Index.

 

For operating procedures, contact the IRVO technical-information desk located in Via Salvador Allende snc Canicattì (AG) tel./fax 0922-734403 E-mail [email protected]; for more information Enol. Gianni Giardina tel. 091- 6278254 E-mail: [email protected].

 

 

 

 

 

RECOMMENDED PERIOD FOR SAMPLE COLLECTION

 

LEAF ANALYSIS	JUNE JULY
TRUNK ANALYSIS	NOVEMBER – DECEMBER – JANUARY – FEBRUARY
SOIL ANALYSIS	MAY – JUNE – SEPTEMBER – OCTOBER – NOVEMBER

 

 

WITHDRAWAL METHODS FOR ELISA TESTS

 

LEAF ANALYSIS                                                                                  

Leaf material

 

Take the leaf sample at the height of the 3rd - 4th leaf starting from the base of the shoot.

Collect approximately 4-6 leaves per plant, place them inside a plastic bag to freeze, close and indicate on the bag the identification data of the company and the plant taken.

Store in the fridge and have it delivered to the analysis laboratory as soon as possible.

We recommend transporting it in a cool bag for those who have to travel long distances.

 

TRUNK ANALYSIS

Woody material: (shoots)

 

Cut the shoot along its entire extension starting from the fruiting cord.

Cut into three pieces of approximately 20 cm

Place in a labeled food bag.

 

SAMPLE COLLECTION METHODS

OF EARTH FOR MICROBIOLOGICAL ANALYSIS (Nematodes)

 

SOIL ANALYSIS  

Xiphinema Index hematology research

 

Coring is a sampling technique for the purpose of chemical-physical and microbiological analyses.

Core barrels are small steel cylinders (about 4 cm in diameter and 40 cm long at most).

You can use iron pipes with dimensions similar to those described above, or a motorized auger or something else that can allow the extraction of a vertical portion of soil

from 0 to 40 cm deep.

Carry out 3 – 4 core samples over the entire area of land, mix the samples and collect everything in a bag.

Quantity: do not exceed 5 kg of final sample. Identify with label, location and company name